article

A practical method for the preparation of total DNA from filamentous fungi

Authors

• • M. I. BORGES
  • Universidade de Brasilia
• • M. O. AZEVEDO
  • Universidade de Brasilia
• • R. BONATELLI JR.
  • Universidade de Brasilia
• • M. S. S. FELIPE
  • Universidade de Brasilia
• • S. ASTOLFI- FILHO
  • Universidade de Brasilia

Abstract

Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. We have developed a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.

Keywords:

How to Cite: BORGES, M. I. , AZEVEDO, M. O. , BONATELLI, R., JR. , FELIPE, M. S. & ASTOLFI- FILHO, S. (1990) “A practical method for the preparation of total DNA from filamentous fungi”, Fungal Genetics Reports. 37(1). doi: https://doi.org/10.4148/1941-4765.1466