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A high molecular mass (about 280 kDa) protein phosphatase 2A holoenzyme was detected in a crude N. crassa extract by gel filtration coupled with phosphatase activity assays. According to Western blot analysis the holoenzyme consists of a 36-kDa catalytic subunit complexed with an additional, yet unidentified, regulatory subunit(s).
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How to Cite: Yatzkan, E. , Dombradi, V. & Yarden, O. (1999) “Detection of a protein phosphatase 2A holoenzyme in Neurospora crassa.”, Fungal Genetics Reports. 46(1). doi: https://doi.org/10.4148/1941-4765.1241