An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker

Wilson Low; Gregory Jedd

doi:10.4148/1941-4765.1091

article

An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker

Authors

- Wilson Low
- National University of Singapore
- Gregory Jedd
- National University of Singapore

Abstract

The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as a selective marker. We mapped the pan-2 fragment to the right arm of LG VI, contig 4, and found that it carries a pan-2 linked gene, atg-12 (NCU10049.3). We report the removal of atg-12 to produce an improved plasmid, pOKE104.

Keywords:

How to Cite: Low, W. & Jedd, G. (2008) “An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker”, Fungal Genetics Reports. 55(1). doi: https://doi.org/10.4148/1941-4765.1091