article

A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea

Authors

• • Xinhua Zhao
  • Purdue University
• • Chaoyang Xue
  • Purdue University
• • Yangseon Kim
  • Purdue University
• • Jin-Rong Xu
  • Purdue University

Abstract

The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed.

Keywords:

How to Cite: Zhao, X. , Xue, C. , Kim, Y. & Xu, J. (2004) “A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea”, Fungal Genetics Reports. 51(1). doi: https://doi.org/10.4148/1941-4765.1137