article

An effect of homology length on gene disruption in Neurospora crassa

Authors

• • Y. Nakanishi
  • Saitama University
• • C. Ishii
  • Saitama University
• • H. Inoue
  • Saitama University

Abstract

For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency.

Keywords:

How to Cite: Nakanishi, Y. , Ishii, C. & Inoue, H. (2005) “An effect of homology length on gene disruption in Neurospora crassa”, Fungal Genetics Reports. 52(1). doi: https://doi.org/10.4148/1941-4765.1120