article

Protocol for the Electroporation of Neurospora Spheroplasts

Authors

• • Gregory O. Kothe
  • SUNY/Buffalo
• • Stephen J. Free
  • SUNY/Buffalo

Abstract

A protocol for electroporating Neurospora strains is described. This protocol uses spheroplasts for transformation rather than germinated conidia with enzyme-weakened walls. High efficiency transformation is obtained with this system even with batches of Novozyme234 which worked poorly in the PEG based protocol.

Keywords:

How to Cite: Kothe, G. O. & Free, S. J. (1996) “Protocol for the Electroporation of Neurospora Spheroplasts”, Fungal Genetics Reports. 43(1). doi: https://doi.org/10.4148/1941-4765.1309