article

A GATEWAY™ Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids

Authors

• • Jeremy R Haag
  • Washington University
• • Dong W Lee
  • Texas A&M University
• • Rodolfo Aramayo
  • Texas A&M University

Abstract

We report the construction of a Destination Vector, called pJHAM007, for the targeted integration of DNA sequences at the histidine-3 (his-3) locus of Neurospora crassa. pJHAM007 has all the necessary features required to perform a simple, rapid and efficient GATEWAY™ recombinational cloning with an Entry Clone to yield a his-3-gene replacement Destination Vector.

Keywords:

How to Cite: Haag, J. R. , Lee, D. W. & Aramayo, R. (2003) “A GATEWAY™ Destination Vector For High-Throughput Construction of Neurospora crassa histidine-3 Gene Replacement Plasmids”, Fungal Genetics Reports. 50(1). doi: https://doi.org/10.4148/1941-4765.1149