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A simple modification of the A. nidulans transformation protocol increases the transformation frequency

Authors

• • J A Cockram
  • University of Hull
• • Sealy-Lewis
  • University of Hull

Abstract

After transformation of Aspergillus nidulans with plasmid DNA the transformants are usually incubated at 37C until transformants appear. We have found that pre-incubation of the transformation plates at room temperature for 24h leads to increased transformation frequencies.

Keywords:

How to Cite: Cockram, J. A. & Sealy-Lewis, . (2000) “A simple modification of the A. nidulans transformation protocol increases the transformation frequency”, Fungal Genetics Reports. 47(1). doi: https://doi.org/10.4148/1941-4765.1220