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Ascospore segregants ("slime"-like) of the triple mutant fz(fuzzy);sg(spontaneous germination) os-1(osmotic) ("slime"; Emerson 1963. Genetica 34:162-182) of Neurospora crassa germinate as a plasmodium which, after some time, results in a morphologically abnormal mycelium. If the mycelium of a "slime"-like isolate is cultured under high osmotic pressure (Nelson et al. 1975. Neurospora Newsl. 22:15-16), it releases cells lacking walls which proliferate as spheroplasts.
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How to Cite: NOVENTA, M. A. , JORGE, J. A. & TERENZI, H. F. (1991) “Role of the cell wall on the expression of osmotic-sensitive (os-1) and temperature-sensitive (cot-1) phenotypes of N. crassa. A comparative study on mycelial and wall-less phenotypes of the "slime" variant”, Fungal Genetics Reports. 38(1). doi: https://doi.org/10.4148/1941-4765.1456