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A simple, rapid procedure for the isolation of DNA for PCR from Gibberella fujikuroi (Fusarium section Liseola)

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Abstract

The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using arbitrarily chosen 10-base primers, one can generate "random amplified polymorphic DNA" (RAPD) markers (Williams et al. 1991 Nucl. Acids Res. 18:6531-6535). These DNA fragments, separated by electrophoresis in an agarose gel, can be used as markers for studying genetic variation within and among fungal populations.

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How to Cite: DuTEAU, N. M. & LESLIE, J. F. (1991) “A simple, rapid procedure for the isolation of DNA for PCR from Gibberella fujikuroi (Fusarium section Liseola)”, Fungal Genetics Reports. 38(1). doi: https://doi.org/10.4148/1941-4765.1451