article

Filtration-enrichment methods for selecting auxotrophs and other mutants in Ascobolus immersus and similar filamentous fungi.

Authors

• • B. C. LAMB
  • Imperial College of Science
• • S. HELMI
  • University of Alexandria

Abstract

Filtration-enrichment is a very successful method for obtaining auxotrophs, but traditional methods cannot be applied directly to many fungi lacking suitable asexual spores, mainly because of a heavy carry-over of parental nutrients into sexual spores. A procedure has been devised to apply filtration-enrichment to such fungi by using hyphal mutagenesis, crossing, germination of sexual spores, fragmentation of germination hyphae into small propagules, and then two cycles of filtration-enrichment in liquid minimal medium (LMM).

Keywords:

How to Cite: LAMB, B. C. & HELMI, S. (1991) “Filtration-enrichment methods for selecting auxotrophs and other mutants in Ascobolus immersus and similar filamentous fungi.”, Fungal Genetics Reports. 38(1). doi: https://doi.org/10.4148/1941-4765.1453