article

Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus

Authors

• • Hei Leung
  • Washington State University
• • Pat Loomis
  • Washington State University
• • Martin L. Pall
  • Washington State University

Abstract

Three transformation systems have been reported for the rice blast fungus Magnaporthe grisea (Parsons et al. 1987 Proc. Natl. Acad. Sci. USA 84:4161-4165; Daboussi et al. 1989 Curr. Genet. 15:453-456; Leung et al. 1990 Curr. Genet. 17:409-411). Among these three selection systems, only hygromycin B resistance provides a dominant selection that can be used for any wild type strain. A second dominant selection marker is needed to transform strains that are already hygromycin B resistant.

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How to Cite: Leung, H. , Loomis, P. & Pall, M. L. (1995) “Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus”, Fungal Genetics Reports. 42(1). doi: https://doi.org/10.4148/1941-4765.1341