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Assessment of Magnaporthe grisea mating type by spore PCR

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Abstract

Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In Saccharomyces cerevisiae, PCR assay can be performed with yeast colonies (Huxley et al. TIG 1990 6:236). Here we describe a PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA.

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How to Cite: Xu, J. & Hamer, J. E. (1995) “Assessment of Magnaporthe grisea mating type by spore PCR”, Fungal Genetics Reports. 42(1). doi: https://doi.org/10.4148/1941-4765.1358