article

A rapid method for isolation of stable niaD and crnA mutants of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae

Authors

• • S. S. Sandhu
  • Osmania University
• • G. Venkateswerlu
  • Osmania University

Abstract

Generally niaD mutants of fungi are selected by spontaneous mutations on appropriate minimal medium supplemented with various concentrations of KClO3 and a nitrogen source (Daboussi et al. 1989 Curr. Genet. 15:453-456; Johnstone et al. 1990 Gene 90:181-192; Malardier et al.1989 Gene 78:147-156; Unkles et al.1989 Gene 78:157-166). But in case of entomopathogenic fungi it has been observed that niaDmutants isolated simply by spontaneous mutation on chlorate were not stable, (Table-1). Therefore a method has been developed to isolate stable niaD mutants of these fungi by treating protoplasts with ethane methane sulfonate (EMS).

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How to Cite: Sandhu, S. S. & Venkateswerlu, G. (1997) “A rapid method for isolation of stable niaD and crnA mutants of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae”, Fungal Genetics Reports. 44(1). doi: https://doi.org/10.4148/1941-4765.1286