article

A simple plating assay for aneuploidy in sexual progeny of Neurospora crassa, and a new allele of mei-1.

Authors

• • A. T. Hagemann
  • University of Oregon
• • J. T. Irelan
  • University of Oregon
• • E. U Selker
  • University of Oregon

Abstract

We developed a simple ascospore plating assay for aneuploidy, based on identifying disomic progeny that inherit two independently selectable mtr alleles. We validated the assay using a known meiotic mutant, mei-2. We used this assay to demonstrate that elevated frequencies of aneuploidy previously reported to be associated with reduced DNA methylation were not, in fact, due to the methylation deficiencies. A new allele of the mei-1 gene was responsible for some of the high aneuploidy.

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How to Cite: Hagemann, A. T. , Irelan, J. T. & Selker, E. U. (1997) “A simple plating assay for aneuploidy in sexual progeny of Neurospora crassa, and a new allele of mei-1.”, Fungal Genetics Reports. 44(1). doi: https://doi.org/10.4148/1941-4765.1275