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The effect of repeated freeze-thaw cycles on cryopreserved Neurospora crassa samples.

Authors

• • Kevin McCluskey
  • University of Missouri- Kansas City
• • Aric Wiest
  • University of Missouri- Kansas City
• • Sheera Walker
  • University of Missouri- Kansas City

Abstract

To better characterize handling parameters for the arrayed mutants prepared for the Neurospora functional genomics program, we have put 7 day old conidia from strain FGSC 2489 through a series of cycles of freezing at -80 C in 25% glycerol and 3.5% reconstituted non-fat dry milk.

Keywords:

How to Cite: McCluskey, K. , Wiest, A. & Walker, S. (2006) “The effect of repeated freeze-thaw cycles on cryopreserved Neurospora crassa samples.”, Fungal Genetics Reports. 53(1). doi: https://doi.org/10.4148/1941-4765.1115